FAQs Automated Thawing System

FAQs Automated Thawing System

FAQs Automated Thawing SystemTroubleshooting your ThawSTAR® Automated Thawing System

  1. Check the condition of the pads by shinning a light into the tube well opening while the unit is off If they appear to be in poor condition the unit may need to be returned for replacement.
  2. Check to see if all the magnets are still installed in the heating blocks. This can be done by shinning a light into the tube well opening while the unit is powered off. There are a total of 4 magnets, 2 on each heat block located about halfway down the heat block on the surfaces that close when a vial is inserted. 
  3. Clean the IR sensor. The IR sensor is located about half way down on the heat blocks. Gently clean it with a Q tip or swab using Isopropyl alcohol. Then perform another thaw to confirm if this resolved the issue.

If the issue remains, please call technical support at 844.627.5577.

Frequently Asked Questions (FAQs)

How do I know my sample is thawed properly when it’s ejected?

When the vial is ejected, a small “pea-size” ice crystal should be visible, and it should disappear within 10-20 seconds with very gentle agitation.  The temperature of the vial contents should be approximately 5-10°C, ensuring that the cells are not subjected to thermal stress.  Note:  Viscous solutions, varying vial volumes, varying media formulations, and vial temperature at insertion may affect the time and appearance of the final thaw.  In some cases, an ice crystal may not be visible or may be larger than pea-size.  In the event of no ice crystal, this should be acceptable as long as the thaw cycle is within the normal time range of 2-3 minutes. If the ice crystal is larger than pea size, gently agitate or “flick” the vial until the ice crystal disappears.

What is the temperature inside the ThawSTAR® Automated Cell Thawing System?

The thawing temperature of the system is designed to produce thermal profiles virtually identical to that seen when using a 37°C water bath thawing method.

Does the airflow around the ThawSTAR Automated Cell Thawing System affect the thawing?

No, airflow from a laminar flow hood or air vent will not affect the temperature sensors inside the instrument or affect its operation.

 

Instrument Performance

Is there any data showing the temperature change of the liquid inside the vial when in the ThawSTAR System?

Yes, this thermal profile data can be found in the ThawSTAR Automated Cell Thawing System White Paper, available for download at www.medcision.com/publications/.

How does the ThawSTAR System address recrystallization concerns?  How can the ThawSTAR System avoid over or under thawing?

Recrystallization occurs when thawing is not performed quickly enough, allowing reformation of ice crystals in the cells. The ThawSTAR System is engineered to deliver a fast, uniform, and reproducible thaw through its patent-pending adaptive sensing technology, which ensures a reproducible thermal profile.

How does the ThawSTAR System determine thaw time for each vial?

The ThawSTAR System determines thaw time based on the initial vial temperature, detected via patent-pending STAR™ technology.  Internal software algorithms ensure an appropriate thaw time to generate a consistent thaw profile.

What is the difference between percent cell recovery and percent cell viability as referred to in the ThawSTAR System White Paper?

Cell recovery is the number of viable cells post-thaw as a percentage of the pre-freeze viable cells.  Cell viability is measured on cells post-thaw using trypan blue exclusion on a hemocytometer.

Instrument Operation

Is it necessary to use the ThawSTAR® Confirmation Vial prior to thawing each day?

ThawSTAR® Confirmation Vials should be frozen and stored in a manner similar to the biospecimens that will be thawed in the ThawSTAR System.  It is recommended to thaw a ThawSTAR Confirmation Vial each time the ThawSTAR System is powered up for use to provide assurance before thawing valuable biospecimens.  For best practices, the use of a confirmation vial at the beginning and end of each thawing session provides assurance that the instrument was performing optimally during the thaw period, which may be recorded in lab notebooks or batch records.

ThawSTAR® Automated Cell Thawing System is optimized for vials with a 1.0 mL fill volume.  What if I have more or less than 1.0 mL in my vial?

The instrument can accommodate slight volume variations around 1.0mL.  If your volumes are greater than +/- 0.1mL, we recommend running a test thaw prior to thawing your biospecimens.