ThawSTAR® Cell Thawing Instrument utilizes patent-pending STAR™ sensing technology to customize the thaw for each vial – whether obtained from liquid nitrogen or -80°C storage.
Post-thaw viability and consistency were evaluated using a total of 6 vials (2 each from 3 donors) containing ex vivo stimulated B cells, were frozen in a -80°C freezer using a CoolCell® cell freezing container and then stored in liquid nitrogen for two weeks. One vial from each donor was removed from LN2, placed into a ThawSTAR® CFT2 Transporter and thawed in a ThawSTAR CFT2 Instrument. The other vial from each donor was removed from LN2 and hand-carried to a water bath. The vials transported and thawed in the ThawSTAR transport and thawing system performed better in terms of cell viability and recovery.
— Data generated by UCSF Diabetes Center and Transplant Surgery
When thawing with the ThawSTAR system, monocyte subset frequencies were modestly higher than for PBMCs thawed using the water bath method (p < 0.01). Percentages also were more aligned with those of freshly isolated cells.
Mean recovery is compared for paired PBMCs isolated immediately after phlebotomy using traditional Ficoll and SepMate methods (left) (n = 6). Mean recovery post cryopreservation is compared for paired PBMCs isolated immediately after phlebotomy for cells thawed in a water bath and using the ThawSTAR CFT2 Instrument (right) (n = 3).
— Data generated by Blood Systems Research Institute
ThawSTAR® AT6 thaw time reproducibility using an optimized algorithm, thaw times are highly reproducible from unit to unit for each fill volume tested and from liquid nitrogen or dry ice temperatures. Thaw times for 17 pre-production AT6 units. 6 ml vials were filled with 2-6 ml of cryopreservation solution, and each volume was thawed in 17 units over 1 week of testing. Thaw times were about 50 s longer when starting from liquid nitrogen temperatures.
“Both of my teams solely use the ThawSTAR® system as their go to thawing device for thawing cells. It is second to none when it comes to efficiency, consistency and sterility. If we have high quality, robust cells, they tend to recover better, propagate better and, overall, perform better in assay. I look forward to using our ThawSTAR® system for many years to come.”
“Often investigators ask how the cells are prepared and thawed. By including the ThawSTAR® method in our SOPs, we inform them of the best way to handle the cells to ensure maximal recovery.”
“The ThawSTAR® system was small, easy to use, and totally automated. It exceeded my expectations. I was happy with how reliable the results were, and how it eliminated the risk of contamination.”
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The white paper provides a detailed description of instrument features, utilization parameters, and performance data. The report also includes new data comparing post-rest recovery of PBMCs thawed with either a ThawSTAR® System or a traditional water bath. These data show significantly higher post-rest recovery of PBMCs thawed using ThawSTAR during the critical 24 hour post-thaw period, suggesting that use of ThawSTAR® Automated Cell Thawing System promotes optimal cell health, potentially improving downstream therapeutic performance.
This white paper describes in detail the MedCision BioT™ ULT Transporter, the first-of-its-kind dry ice-based portable cooling system that maintains a stable – 75° to -50°C environment for over 24 hours on one charge of dry ice, enabling reliable handling and transfer of valuable temperature-sensitive biospecimens as part of a larger cold chain standardization process.